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Wham is suitable for paired-conclude Illumina libraries with regular insert measurements (~300bp-500bp). Wham integrates mate-pair mapping, split read mapping, comfortable-clipping, alternative alignment and consensus sequence primarily based proof to forecast SV breakpoints with solitary-nucleotide precision. Wham generates a put together pileup (
Wham clusters each of the prospect breakpoints and rounds their positions to the closest tenth base pair. The placement with the very best examine assistance is described. Should the delicate-clipped consensus sequence can be aligned for the putative breakpoint location utilizing the Smith-Waterman algorithm, the breakpoint is further refined to th